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Chemical reagents, kits, columns, and other products used for DNA synthesis, and to subsequently run purification columns; includes genetic assembly systems.
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The 5' DNA adenylation Kit is a simple and efficient enzymatic method for generating 5' -adenylated DNA. The kit is optimized to produce the adenylated DNA with or without 3' -terminator. The 5 DNA adenylation kit routinely generates greater than 95% conversion of pDNA to AppDNA. This highly efficient process eliminates the need for gel isolation of the product and increases overall yield.
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PURExpress (aa, tRNA) Kit is a variation of the PURExpress In vitro Protein Synthesis Kit where the amino acids and tRNA are omitted from the translation mix.
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DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3' to 5' exonuclease activity, but has lost 5' to 3' exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
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The Ph.D. Peptide Display Cloning System contains M13KE vector, the parent vector of NEB's Ph.D. libraries. The included M13 extension primer is used to make DNA duplex out of a user provided synthetic oligo, with or without random bases. The display system is best used to display small peptides, i.e. sequences of 50 amino acids or less.
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